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Yeast artificial chromosomes employed for random assembly of biosynthetic pathways and production of diverse compounds in Saccharomyces cerevisiae

Michael Naesby1, Søren VS Nielsen1, Curt AF Nielsen1, Trine Green1, Thomas Ø Tange1, Ernesto Simón1, Philipp Knechtle1, Anders Hansson1, Markus S Schwab1, Olca Titiz1, Christophe Folly1, Roberto E Archila1, Milena Maver1, Stephan van Sint Fiet1, Thiamo Boussemghoune1, Michael Janes1, A SS Kumar2, Shailendra P Sonkar2, Partha P Mitra2, V AK Benjamin2, Nimitha Korrapati2, Inala Suman2, Esben H Hansen3, Tanja Thybo3*, Neil Goldsmith1 and Alexandra S Sorensen1

1 Evolva SA, Hagmattstrasse 6, 4123 Allschwil, Switzerland

2 Evolva Biotech Private Limited, 203-206, 2nd Floor, Tara Tycoon Building, Tarnaka, Secunderabad 500 017, India

3 Evolva A/S, Bülowsvej 25, 1870 Frederiksberg C, Denmark

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Microbial Cell Factories 2009, 8:45 doi:10.1186/1475-2859-8-45

Published: 13 August 2009

Additional files

Additional file 1:

Spectral data of detected compounds. Spectral data.

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Additional file 2:

Compounds produced from halogenated precursors. Ion chromatograms.

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Additional file 3:

Halogenated flavanones. Ion chromatograms.

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Additional file 4:

Promoter and Terminator sequence names and accession numbers. Overview of names and accession numbers.

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Additional file 5:

Vectors used for preparing eYACs. Vector diagrams.

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Additional file 6:

FL1 library enzyme names and accession numbers. Overview of names and accession numbers.

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Additional file 7:

FL2 library enzyme names and accession numbers. Overview of names and accession numbers.

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Additional file 8:

Fragmentation patterns for identification of flavonoids. Diagrams for fragmentation of flavonoid molecules.

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Additional file 9:

Compounds produced by FL1 pathway. Ion chromatograms.

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Additional file 10:

Compounds produced by FL2 pathway. Ion chromatograms.

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Additional file 11:

Supplementary references. References.

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