Table 2

Fungal entities isolated from bioreactors at the beginning and at the end of the biotreatment

3 days
 120 days
Entities
 A
 B
 C
 D
 A
 B
 C
 D
Acremonium curvulum W. Gams



50 (0.4%)


Aspergillus foetidus Thom & sRaper
50 (0.5%)





Aspergillus fumigatus Fresenius var. fumigatus
 100 (0.6%)
 100 (1.0%)

50 (0.4%)


Aspergillus ochraceus K. Wilhelm



100 (0.9%)


Aspergillus ustus (Bainier) Thom & Church

211,5 (0.7%)




Basidiomycete with clamp connections
 450 (2.5%)
 300 (3.0%)
 5499 (17.2%)
 4849 (18.8%)



Basidiomycete with clamp connections and arthroconidia

211,5 (0.7%)




Basidiomycete with clamp connections and chlamidospores


776 (3.0%)



DBB+ avellaneous basidiomycete with arthroconidia

211,5 (0.7%)




DBB+ dematiaceous basidiomycete
 50 (0.3%)
423 (1.3%)
 194 (0.8%)



DBB+ ialinus basidiomycete with arthroconidia
 250 (1.4%)
 100 (1.0%)
 634.5 (2.0%)
 388 (1.5%)



DBB+ ialinus basidiomycete with chlamidospores
 100 (0.6%)
 100 (1.0%)





DBB+ ialinus basidiomycete morphotype 1
100 (1.0%)





DBB+ ialinus basidiomycete morphotype 2
 800 (4.5%)
 750 (7.5%)
 5710.5 (17.9%)
 3685 (14.3%)



DBB+ ialinus basidiomycete morphotype 3

423 (1.3%)




Beauveria bassiana (Balsamo) Vuillemin





306 (3.6%)
Cladosporium chlorocephalum (Fresenius) E.W. Mason & M.B. Ellis
 500 (2.8%)
 200 (2.0%)
 846 (2.6%)
 1357.5 (5.3%)



Cladosporium herbarum (Persoon) Link
 650 (3.7%)
 50 (0.5%)
 1480.5 (4.6%)
 388 (1.5%)



Clitocybe sp.

211.5 (0.7%)




Engyodontium album (Limber) de Hoog

211.5 (0.7%)




Eurotium herbariorum (F.H. Wiggers: Fries) Link
 50 (0.3%)






Fusarium solani f. pisi (Jones) Snyder & Hansen



50 (0.4%)


Geomyces pannorum (Link) Sigler & J.W. Carmichael var. pannorum





306 (3.6%)
Microsporum gypseum (E. Bodin) Guiart & Grigoraki



50 (0.4%)


Myrothecium roridum Tode

211.5 (0.7%)




Penicillium aculeatum Raper & Fennel
 50 (0.3%)






Penicillium aurantiogriseum Dierckx
 50 (0.3%)
634.5 (2.0%)
 1745.5 (6.8%)



Penicillium camemberti Thom
 100 (0.6%)






Penicillium chrysogenum Thom
 1350 (7.6%)
 400 (4.0%)
 4864.5 (15.2%)
 582 (2.3%)
 50 (0.4%)
6430 (75.0%)
Penicillium commune Thom

211.5 (0.7%)




Penicillium crustosum Thom


388 (1.5%)



Penicillium digitatum (Persoon) Saccardo






1160 (12.5%)
Penicillium glabrum (Wehmer) Westling
 1650 (9.3%)
 250 (2.5%)
 211.5 (0.7%)
 1357.5 (5.3%)
 50 (0.4%)

1160 (12.5%)
Penicillium palitans Westling
 100 (0.6%)
2538 (7.9%)




Penicillium paneum Frisvad (ined.)



50 (0.4%)


Penicillium paxilli Bainier

211.5 (0.7%)




Penicillium polonicum K.M. Zalessky
 250 (1.4%)
211.5 (0.7%)




Penicillium purpurescens Raper & Thom
 550 (3.1%)




306 (3.6%)
Penicillium restrictum J.C. Gilman & E.V. Abbott
 150 (0.8%)






Penicillium solitum Westling


2133.5 (8.3%)


2320 (25.0%)
Penicillium spinulosum Thom
 900 (5.1%)






Penicillium waksmanii K.M. Zalessky
 50 (0.3%)
 50 (0.5%)
1164 (4.5%)



Scedosporium apiospermum (Saccardo) Saccardo
 9400 (53.1%)
 7500 (75.4%)
 6557 (20.5%)
 6400 (24.8%)
 11250 (96.2%)
 16150 (100%)
 1225 (14.3%)
 4641 (50.0%)
Schizophyllum commune Fries
 50 (0.3%)

194 (0.8%)



DBB-dematiaceous sterile mycelium
 50 (0.3%)






DBB-moniliaceous sterile mycelium
 50 (0.3%)
211.5 (0.7%)
 194 (0.8%)



Total CFU g(dw)-1 (mean ± st.dev)
 17700 ± 6199 a*
 9950 ± 3913 b*
 31937 ± 17655 c*
 25796 ± 14905 ac*
 11700 ± 5371 a*
 16150 ± 6277 b*
 8573 ± 15568 c*
 9281 ± 21817 c*

PCBs removal in mg kg-1 (mean ± st.dev)



65 ± 4
 28 ± 3
 129 ± 8
 74 ± 50

Fungal entities are expressed in number of colony forming units per g of soil dry weight (CFU g-1dw). In brackets is indicated the percentage of presence of the given species with respect to the total CFU g-1dw. A = Bioaugmented solid-phase bioreactors, B = Enzyveba-free solid-phase bioreactors, C = bioaugmented slurry-phase bioreactors, D = Enzyveba-free slurry-phase bioreactors. "DBB + or -" means positive or negative to the reaction with Diazonium Blue B salts. Different small letters indicate significant differences among fungal loads; * indicates significant differences among fungal loads after 3 days and after 120 days (Mann Whitney, P = 0.05). PCB depletion occurs in bioreactors is also reported.

Tigini et al. Microbial Cell Factories 2009 8:5   doi:10.1186/1475-2859-8-5