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Bacillus amyloliquefaciens GA1 as a source of potent antibiotics and other secondary metabolites for biocontrol of plant pathogens

Anthony Arguelles-Arias1, Marc Ongena2, Badre Halimi3, Yannick Lara4, Alain Brans1, Bernard Joris1 and Patrick Fickers1*

Author Affiliations

1 Centre for Protein Engineering, Physiology and Bacterial Genetics, University of Liege, 4000 Liege, Belgium

2 Walloon Centre for Industrial Biology, Bio-Industry Unit, Gembloux Agro-Bio Tech, University of Liege, B-5030 Gembloux, Belgium

3 Walloon Centre for Industrial Biology, Microbial Technology Unit, University of Liege, 4000 Liege, Belgium

4 Centre for Protein Engineering, Cyanobacteria Group, University of Liege, 4000 Liege, Belgium

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Microbial Cell Factories 2009, 8:63  doi:10.1186/1475-2859-8-63

Published: 26 November 2009

Abstract

Background

Phytopathogenic fungi affecting crop and post-harvested vegetables are a major threat to food production and food storage. To face these drawbacks, producers have become increasingly dependent on agrochemicals. However, intensive use of these compounds has led to the emergence of pathogen resistance and severe negative environmental impacts. There are also a number of plant diseases for which chemical solutions are ineffective or non-existent as well as an increasing demand by consumers for pesticide-free food. Thus, biological control through the use of natural antagonistic microorganisms has emerged as a promising alternative to chemical pesticides for more rational and safe crop management.

Results

The genome of the plant-associated B. amyloliquefaciens GA1 was sample sequenced. Several gene clusters involved in the synthesis of biocontrol agents were detected. Four gene clusters were shown to direct the synthesis of the cyclic lipopeptides surfactin, iturin A and fengycin as well as the iron-siderophore bacillibactin. Beside these non-ribosomaly synthetised peptides, three additional gene clusters directing the synthesis of the antibacterial polyketides macrolactin, bacillaene and difficidin were identified. Mass spectrometry analysis of culture supernatants led to the identification of these secondary metabolites, hence demonstrating that the corresponding biosynthetic gene clusters are functional in strain GA1. In addition, genes encoding enzymes involved in synthesis and export of the dipeptide antibiotic bacilysin were highlighted. However, only its chlorinated derivative, chlorotetaine, could be detected in culture supernatants. On the contrary, genes involved in ribosome-dependent synthesis of bacteriocin and other antibiotic peptides were not detected as compared to the reference strain B. amyloliquefaciens FZB42.

Conclusion

The production of all of these antibiotic compounds highlights B. amyloliquefaciens GA1 as a good candidate for the development of biocontrol agents.