Figure 1.

Construction of the recombinant P. pastoris X-33 strain. (A) The IP gene and polypeptide sequence. The bent arrows indicate the ends of the IP gene and polypeptide. The tripeptide linking the B and A chains is underlined. The nucleotides comprising the 5' and 3' restriction sites are shown in italics. The A and B chain aa residue numbers carry the cognate 'A' and 'B' prefixes, respectively. The black triangles denote the sites of trypsin cleavage. (B) Map of the yeast integrative vector encoding IP. The IP is cloned between the XhoI site in the α-factor signal encoding sequence and the NotI site in the polylinker.