Table 1

Insulin precursor production with P. pastoris

Strain (copy#)

MeOH

(%)

Induction time

(h)

Final biomass

(g L^-1)

Product ^a

(g L^-1)

Culture supernatant

Product ^b

(g L^-1)

Culture broth

Mass

Reference ^c

Mut^+/s (6-8)

nd ^d

87

nd

1.5

nd

nd

[26]

Mut^s (nd)

1.0

72

80 to 90

0.22

nd

nd

[25]

Mut^s (11)

<0.1

110

89

0.3

nd

MS ^e

[24]

Mut^s (11)

1 to 0.5

136

109

0.25

nd

MS

[23]

Mut^s (nd)

nd

72

nd

3.6

1.47

nd

[27]

Mut⁺ (12)

0.8

96

nd

0.18

nd

nd

[28]

Mut⁺ (~2) ^f

0.2

88

59

3.84

3.075

MS

Present study

^a Concentrations were determined by reverse phase HPLC from clarified culture supernatant

^b Concentrations calculated considering the volumetric portion of biomass in the culture broth

^c Only references on secretory insulin precursor production with P. pastoris included containing respective quantitative data

^d nd; not determined

^e MS; mass spectrometry

^f Copy number not experimentally determined. Survival at zeocin concentrations up to 0.5 mg mL^-1 indicates a copy number of 2 [41].

Gurramkonda et al. Microbial Cell Factories 2010 9:31   doi:10.1186/1475-2859-9-31