Shake flask fed-batch culture growth of E. coli RV308/pibpfxsT7lucA/pCTUT7MBP-RI with and without target protein production. (A) Growth curves (OD600) of independent fed-batch cultivations at 37°C and the calculated corresponding specific growth rate μ (B). Induction of 6xHis-MBP-RI synthesis was performed at the selected time points when the specific growth rate was about 0.33 h-1, 0.22 h-1, or 0.1 h-1, respectively. The culture cultivation curves were generated from three independent cultivation experiments.
Šiurkus et al. Microbial Cell Factories 2010 9:35 doi:10.1186/1475-2859-9-35