Figure 6.

Chart A: amounts of soluble 6 × His-MBP-RI protein in mg per gram of cell dry weight (mg gCDW-1) after shake flask batch and fed-batch cultivation with 6 × His-MBP-RI protein production at 37°C in E. coli RV308/pibpfxsT7lucA/pCTUT7MBP-RI. Columns A1, A2 and A3 represent soluble 6 × His-MBP-RI protein amounts [mg (gCDW)-1 ] obtained after 4 hours of fed-batch production at 37°C when induction was performed at different specific growth rates (bars A1-A3). Bars A4-A6 represent the amounts of soluble 6 × His-MBP-RI protein [mg (g CDW)-1] after 4 hours of batch production at 37°C in MSM medium containing 10 g L-1 of glucose (column A4), semi-synthetic medium containing 10 g L-1 of glucose (column A5) or LB medium (column A6). Chart B: ribonuclase inhibitor activities (in kilo-units), detected in the cellular crude extracts, calculated for 1 gram of cell wet weight [kU (gCWW)-1] after shake flask batch and fed-batch cultivation with 6 × His-MBP-RI protein production at 37°C in E. coli RV308/pibpfxsT7lucA/pCTUT7MBP-RI. Columns B1, B2 and B3 represent ribonuclase inhibitor activities obtained after 4 hours of fed-batch production at 37°C when induction was carried at different specific growth rates (bars B1-B3). Bars B4-B4 show ribonuclase inhibitor activities after 4 hours of 6 × His-MBP-RI protein batch production at 37°C in MSM medium containing 10 g L-1 of glucose (column B4), semi-synthetic medium containing 10 g L-1 of glucose (column B5) or LB medium (column B6).

Šiurkus et al. Microbial Cell Factories 2010 9:35   doi:10.1186/1475-2859-9-35
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