Table 1

Bacterial lysis methods for IB purification.

Lysis method

IB protein

DNase

Detergents

Tested viability

Reference


NON MECHANICAL LYSIS METHODS

LYSOZYME

LACVP1

Yes

Yes

No

[19-22]


VP1LAC

Yes

Yes

No

[7-9,19-27]


V2LAC

Yes

Yes

No

[22]


TSP

Yes

Yes

No

[23]


VP1GFP

Yes

Yes

No

[7,28-30]


hDHFR

Yes

Yes

No

[7]


Aβ42-BFP

Yes

Yes

No

[7]


β-lactamase

Yes

Yes

No

[31]


Prochymosin

No

Yes

No

[32]


HET-s fungal prion

Yes

Yes

No

[33]


Aβ42-GFP

Yes

Yes

No

[30,34]


Aβ42-BFP

Yes

Yes

No

[30]


MalE-Bla and MalE31-Bla

No

No

No

[35]


MalE-PhoA and MalE31-PhoA

No

No

No

[35]


NON-IONIC DETERGENTS

CBDclosN-SAA

No

Yes

No

[36]


SAA-6HisC

No

Yes

No

[36]


Maltodextrin phosphorylase

No

Yes

No

[37]


CBDclosSabA

No

Yes

No

[38]


MECHANICAL LYSIS METHODS

HOMOGENIZER

rhBMP-2

No

Yes

No

[17,39]


G-CSF

No

No

No

[40]


rhMCSF

No

No

No

[41]


rHEWL

No

Yes

No

[42]


GFP

No

No

No

[43]


FRENCH PRESS

EGD

No

No

No

[44]


TvDAO

No

Yes

No

[45,46]


NS3 protein

No

Yes

No

[47]


SONICATION

IFN-α

No

No

No

[48]


β-galactosidase

No

No

No

[49]


pGH

No

No

No

[50]


Pre-β-lactamase

No

No

No

[51]


Procathepsin B

No

Yes

No

[52]


COMBINED LYSIS METHODS

SONICATION

+

HOMOGENIZER

Npro fusion proteins

No

Yes

No

[53]


SONICATION

+

LYSOZYME

or

LYSOZYME

+

SONICATION

Prochymosin

No

Yes

Yes

[54]


Cro-β-gal

Yes

Yes

Yes

[55]


His-GST-GFP

Yes

No

No

[56]


Prochymosin B

No

Yes

No

[54]


CLIPB14 Serine protease

Yes

Yes

No

[57]


FRENCH PRESS

+

LYSOZYME

or

LYSOZYME

+

FRENCH PRESS

PHA synthase

Yes

Yes

No

[58]


Class II PHA synthase

Yes

Yes

No

[58]


Rodríguez-Carmona et al. Microbial Cell Factories 2010 9:71   doi:10.1186/1475-2859-9-71

Open Data