pH-controlled cultivation of E. coli K12 in minimal media supplemented with 10 g/L glucose. (A) Cultivation with pH-control in microfluidic MTP and in 2 L stirred tank reactor (working volume of 1 L) at a matched kLa-value of 460 1/h. The time-axis shows an offset of 1.2 h due to different duration of the lag-phases in both cultivations performed independently from each other. (B) Reference cultivation without pH-control in microfluidic MTP. (C) Dosage of 2 M ammonium solution and 1 M phosphoric acid during the pH-control in microfluidic MTP and stirred tank reactor.
Funke et al. Microbial Cell Factories 2010 9:86 doi:10.1186/1475-2859-9-86