Figure 6.

Fed-batch cultivations of E. coli K12 in minimal media initially supplemented with 5 g/L glucose. The scattered light and DCW are shown in black, the pH-value and the DOT in red and blue, respectively. The feed solution was composed of 500 g/L glucose and 70 g/L (NH4)2HPO4. All cultivations were carried out at a kLa-value of 460 1/h (A) linear feeding in microfluidic MTP with 2 mg/h (= 4 g/L/h with reference to 500 μL initial culture volume) (B) exponential feeding in microfluidic MTP with μset = 0.2 1/h (C) exponential feeding in 2 L stirred tank reactor with μset = 0.2 1/h

Funke et al. Microbial Cell Factories 2010 9:86   doi:10.1186/1475-2859-9-86
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