The thermostability of recombinant FumF protein. The enzyme was pre-incubated at temperature ranging from 15 to 70°C at optimum pH. Subsequently, the residual activity was determined with fumarate as the substrate at 55°C in 100 mM glycine/NaOH buffer (pH 8.5).
Jiang et al. Microbial Cell Factories 2010 9:91 doi:10.1186/1475-2859-9-91