Microbial Cell Factories - Latest Comments

Correction

José Flores-Uribe — 2012-01-04T09:20:51Z

This isn't the first microalga PHB production report. In 2010 Chaogang modified Chlamydomonas reinhardtii with phbB and phbC to produce PHB
BIOSYNTHESIS OF POLY-3-HYDROXYBUTYRATE (PHB) IN THE TRANSGENIC GREEN ALGA CHLAMYDOMONAS REINHARDTII

Comments to: MS: 4658954235171682

Grzegorz Wegrzyn — 2011-12-19T11:23:12Z

Almost everything is OK. The only thing I may discuss is the problem with insertion of the name of the organism (Escherichia coli) in Abstract. If it is possible, I would suggest to introduce this name, as suggested at the proof stage. This would not change the merit of the paper, but will allow to avoid any potential confusions of readers about the bacterial species investigated in this work.

Correction of legends of Figs 8 and 9

Peter Neubauer — 2011-07-19T11:11:49Z

In this article legend of Figure 8 is mixed up with the legend of Figure 9. The right text is:

Figure 8: RI activities in the total soluble protein fraction (white bars) and in the periplasmic fraction (grey bars) of E. coli of RV308 pCUlac-pelB-RI and RV308 pCUlac-His6-RI from samples of batch and fed-batch bioreactor cultivations without and with addition of DTT. Data derive from three activity assays.

Figure 9: The amount of reduced DTT during batch shake flask and fed-batch bioreactor processes with microarerobic production mode, was measured by using Measure-iTTM Kit (Invitrogen). The samples for reduced DTT evaluation were taken every synthesis hour starting with DTT addition moment. The data is derived from three assays.

Correcture of legend for Fig. 1

Peter Neubauer — 2011-02-08T21:01:11Z

In this article Figures 1 B and C are mixed up in the figure legend. The right text is:
"Figure 1: Presentation of different cultivation modes used in this study. (A) Scheme of the two reactor setups used in the study, a standard fed-batch in a stirred tank reactor and a fed-batch performed in a STR-PFR two-compartment reactor system with feeding of the glucose feed solution into the entrance of the PFR. (B-D) illustrate typical cultivation data of E. coli W3110 with the different types of cultivation applied in this study. (C) Glucose limited fed-batch cultivation with continuous feed of glucose at a
constant feed rate in a single STR (reference). (B) Same as (C) but with a downshift of the DOT by a simultaneous decrease of stirrer speed and aeration rate. (D) Cultivation in a STR-PFR system with feeding of the glucose feed solution into the lower part of the PFR. The graphs show the experimental data for cell density (OD600), glucose concentration, and the DOT. All analyses from the STR-PFR system were performed from samples collected by the standard sampling valve in the STR. Zero hours indicates the time of the feed start. The analyses in (D) reflect samples taken from the STR compartment. All cultivations were started with a glucose concentration of 40 g L-1."

Erratum

Ahmad Zeidan — 2011-02-08T21:00:13Z

Please note the following erratum in equations 7 & 8:

The first “minus” sign in both equations should be a “plus” sign

This trehalose synthase is the family GH13 member

Stefan Janecek — 2009-07-18T17:31:44Z

There is an error in this paper concerning the classification of the new trehalose synthase from Enterobacter hormaechei. The authors wrote their trehalose synthase belongs to the glycoside hydrolase family GH16 (the alignment shown in Figure 1), indicating also the essential residues. But it is clear that the sequence features (Fig. 1) are those of the family GH13 (the alpha-amylase family, forming the clan GH-H - the families GH13, GH70 and GH77). Obviously the authors ignored the key references, such as the sequence-based CAZy classification of GHs (http://www.cazy.org/) as well as the relevant reviews on the alpha-amylase family GH13 (clan GH-H) describing sequence/structure/function/evolution of these enzymes.

Correction of an erratum in the reference list

Maria Klapa — 2008-05-22T17:26:09Z

The correct reference 22 is :

de Graaf AA, Striegel K, Wittig RM, Laufer B, Schmitz G, Wiechert W, Sprenger GA, Sahm H.: Metabolic state of Zymomonas mobilis in glucose-, fructose-, and xylose-fed continuous cultures as analysed by 13C- and 31P-NMR spectroscopy. Arch Microbiol 1999, 171:371-385

Errata

Pau Ferrer — 2007-11-19T11:01:37Z

Please notice the following errata:

1. In the Conclusion section, last line of the last paragraph, the following sentence:

..., which also triggers UPR [12].

should read:

..., which also triggers UPR [10].

2. In the Methods section Strains, line 6, the following sentence:

...[13] co-transformed with the vector pPICZFLDa ROL....

should read:

...[14] co-transformed with the vector pPICZFLDa ROL....