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1.

1085
Accesses

Review   Open Access Highly Accessed

Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli

Hans Sørensen, Kim Mortensen Microbial Cell Factories 2005, 4:1 (4 January 2005)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

2.

1038
Accesses

Research   Open Access Highly Accessed

Genome, secretome and glucose transport highlight unique features of the protein production host Pichia pastoris

Diethard Mattanovich, Alexandra Graf, Johannes Stadlmann, Martin Dragosits, Andreas Redl, Michael Maurer, Martin Kleinheinz, Michael Sauer, Friedrich Altmann, Brigitte Gasser Microbial Cell Factories 2009, 8:29 (2 June 2009)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

3.

1001
Accesses

Research   Open Access

Artificial biosynthesis of phenylpropanoic acids in a tyrosine overproducing Escherichia coli strain

Sun-Young Kang, Oksik Choi, Jae Lee, Bang Hwang, Tai-Boong Uhm, Young-Soo Hong Microbial Cell Factories 2012, 11:153 (3 December 2012)

Abstract | Full text | PDF | PubMed

4.

955
Accesses

Research   Open Access Highly Accessed

Microalgae as bioreactors for bioplastic production

Franziska Hempel, Andrew S Bozarth, Nicole Lindenkamp, Andreas Klingl, Stefan Zauner, Uwe Linne, Alexander Steinbüchel, Uwe G Maier Microbial Cell Factories 2011, 10:81 (17 October 2011)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central | 1 comment |  Editor’s summary

Solar-powered polyester production is proved feasible by causing microalgae to express enzymes borrowed from specialized bacteria, to result in biosynthesis of poly-3-hydroxybutyrate.

5.

805
Accesses

Review   Open Access Highly Accessed

Strategies for successful recombinant expression of disulfide bond-dependent proteins in Escherichia coli

Ario de Marco Microbial Cell Factories 2009, 8:26 (14 May 2009)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

6.

807
Accesses

Review   Open Access Highly Accessed

Potential and utilization of thermophiles and thermostable enzymes in biorefining

Pernilla Turner, Gashaw Mamo, Eva Karlsson Microbial Cell Factories 2007, 6:9 (15 March 2007)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

7.

784
Accesses

Technical Notes   Open Access

A versatile, efficient strategy for assembly of multi-fragment expression vectors in Saccharomyces cerevisiae using 60-bp synthetic recombination sequences

Niels GA Kuijpers, Daniel Solis-Escalante, Lizanne Bosman, Marcel van den Broek, Jack T Pronk, Jean-Marc Daran, Pascale Daran-Lapujade Microbial Cell Factories 2013, 12:47 (10 May 2013)

Abstract | Provisional PDF | PubMed

8.

754
Accesses

Research   Open Access

Construction of Escherichia coli strains with chromosomally integrated expression cassettes for the synthesis of 2′-fucosyllactose

Florian Baumgärtner, Lyudmila Seitz, Georg A Sprenger, Christoph Albermann Microbial Cell Factories 2013, 12:40 (1 May 2013)

Abstract | Full text | PDF | ePUB | PubMed

9.

709
Accesses

Review   Open Access Highly Accessed

Microbial production of hyaluronic acid: current state, challenges, and perspectives

Long Liu, Yanfeng Liu, Jianghua Li, Guocheng Du, Jian Chen Microbial Cell Factories 2011, 10:99 (16 November 2011)

Abstract | Full text | PDF | PubMed

10.

693
Accesses

Research   Open Access

Modification of glucose import capacity in Escherichia coli: physiologic consequences and utility for improving DNA vaccine production

Laura G Fuentes, Alvaro R Lara, Luz M Martínez, Octavio T Ramírez, Alfredo Martínez, Francisco Bolívar, Guillermo Gosset Microbial Cell Factories 2013, 12:42 (2 May 2013)

Abstract | Full text | PDF | ePUB | PubMed

11.

692
Accesses

Research   Open Access

Stable expression plasmids for Streptomyces based on a toxin-antitoxin system

Laura Sevillano, Margarita Díaz, Ramón I Santamaría Microbial Cell Factories 2013, 12:39 (25 April 2013)

Abstract | Full text | PDF | ePUB | PubMed

12.

684
Accesses

Research   Open Access Highly Accessed

Recombinant protein expression in Pichia pastoris strains with an engineered methanol utilization pathway

Florian W Krainer, Christian Dietzsch, Tanja Hajek, Christoph Herwig, Oliver Spadiut, Anton Glieder Microbial Cell Factories 2012, 11:22 (13 February 2012)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

13.

675
Accesses

Research   Open Access Highly Accessed

New insights into Escherichia coli metabolism: carbon scavenging, acetate metabolism and carbon recycling responses during growth on glycerol

Karla Martínez-Gómez, Noemí Flores, Héctor M Castañeda, Gabriel Martínez-Batallar, Georgina Hernández-Chávez, Octavio T Ramírez, Guillermo Gosset, Sergio Encarnación, Francisco Bolivar Microbial Cell Factories 2012, 11:46 (18 April 2012)

Abstract | Full text | PDF | PubMed

14.

670
Accesses

Research   Open Access

Morphology engineering - Osmolality and its effect on Aspergillus niger morphology and productivity

Thomas Wucherpfennig, Timo Hestler, Rainer Krull Microbial Cell Factories 2011, 10:58 (29 July 2011)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

15.

628
Accesses

Technical Notes   Open Access

Sandwich ELISA for quantitative detection of human collagen prolyl 4-hydroxylase

Ekaterina Osmekhina, Antje Neubauer, Katharina Klinzing, Johanna Myllyharju, Peter Neubauer Microbial Cell Factories 2010, 9:48 (17 June 2010)

Abstract | Full text | PDF | PubMed

16.

628
Accesses

Research   Open Access

Use of a Tn5-based transposon system to create a cost-effective Zymomonas mobilis for ethanol production from lignocelluloses

Xi Zhang, Tianyv Wang, Wen Zhou, Xianghui Jia, Haoyong Wang Microbial Cell Factories 2013, 12:41 (2 May 2013)

Abstract | Provisional PDF | PubMed

17.

624
Accesses

Commentary   Open Access Highly Accessed

Towards universal systems for recombinant gene expression

Hans Sørensen Microbial Cell Factories 2010, 9:27 (30 April 2010)

Abstract | Full text | PDF | PubMed

18.

598
Accesses

Research   Open Access Highly Accessed

The intra- and extracellular proteome of Aspergillus niger growing on defined medium with xylose or maltose as carbon substrate

Xin Lu, Jibin Sun, Manfred Nimtz, Josef Wissing, An-Ping Zeng, Ursula Rinas Microbial Cell Factories 2010, 9:23 (20 April 2010)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

19.

595
Accesses

Review   Open Access Highly Accessed

Microalgal biofactories: a promising approach towards sustainable omega-3 fatty acid production

T Adarme-Vega, David K Y Lim, Matthew Timmins, Felicitas Vernen, Yan Li, Peer M Schenk Microbial Cell Factories 2012, 11:96 (25 July 2012)

Abstract | Full text | PDF | PubMed

20.

590
Accesses

Research   Open Access

Production of functionalized polyhydroxyalkanoates by genetically modified Methylobacterium extorquens strains

Philipp Höfer, Young J Choi, Michael J Osborne, Carlos B Miguez, Patrick Vermette, Denis Groleau Microbial Cell Factories 2010, 9:70 (16 September 2010)

Abstract | Full text | PDF | PubMed

21.

577
Accesses

Research   Open Access

Lactic acid fermentation as a tool to enhance the functional features of Echinacea spp

Carlo Giuseppe Rizzello, Rossana Coda, Davinia Sánchez Macías, Daniela Pinto, Barbara Marzani, Pasquale Filannino, Giammaria Giuliani, Vito Michele Paradiso, Raffaella Di Cagno, Marco Gobbetti Microbial Cell Factories 2013, 12:44 (4 May 2013)

Abstract | Provisional PDF | PubMed

22.

574
Accesses

Review   Open Access Highly Accessed

Production of recombinant proteins in E. coli by the heat inducible expression system based on the phage lambda pL and/or pR promoters

Norma A Valdez-Cruz, Luis Caspeta, Néstor O Pérez, Octavio T Ramírez, Mauricio A Trujillo-Roldán Microbial Cell Factories 2010, 9:18 (19 March 2010)

Abstract | Full text | PDF | PubMed

23.

569
Accesses

Research   Open Access

Enhancement of thioredoxin/glutaredoxin-mediated L-cysteine synthesis from S-sulfocysteine increases L-cysteine production in Escherichia coli

Takeshi Nakatani, Iwao Ohtsu, Gen Nonaka, Natthawut Wiriyathanawudhiwong, Susumu Morigasaki, Hiroshi Takagi Microbial Cell Factories 2012, 11:62 (18 May 2012)

Abstract | Full text | PDF | PubMed

24.

567
Accesses

Review   Open Access Highly Accessed

Microbial factories for recombinant pharmaceuticals

Neus Ferrer-Miralles, Joan Domingo-Espín, José Corchero, Esther Vázquez, Antonio Villaverde Microbial Cell Factories 2009, 8:17 (24 March 2009)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

25.

565
Accesses

Research   Open Access

Optimizing heterologous protein production in the periplasm of E. coli by regulating gene expression levels

Susan Schlegel, Edurne Rujas, Anders Jimmy Ytterberg, Roman A Zubarev, Joen Luirink, Jan-Willem de Gier Microbial Cell Factories 2013, 12:24 (12 March 2013)

Abstract | Full text | PDF | PubMed

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